Retinoic acid induces PGI-synthase expression in human endothelial cells.

2008, J Lipid Res 2008;49:1707-1714
Camacho M, Rodríguez C, Salazar J, Martínez-González J, Ribalta J, Escudero JR, Vila L.
Autors del centre relacionats: Martínez-González Jose, Rodríguez Cristina.

Retinoic acid (RA) exhibits anti-inflammatory, anti-tumor, and immuno-modulatory actions, and affects angiogenesis and thrombosis. Arachidonic acid (AA) metabolites are involved in all these processes. We explored the effect of RA on AA metabolism in human umbilical vein endothelial cells (HUVEC). 13-cis-RA increased the release of PGI2, both spontaneous and thrombininduced, in terms of 6-oxo-PGF1 analyzed by enzyme-immunoassay. Co-incubation with 13-cis-RA and IL-1 resulted in a synergic increase in the release of PGI2. Consistently, 13-cis-RA increased the ability of HUVEC to inhibit AA-induced platelet aggregation. 13-cis-RA did not induce COX-isoenzyme expression, determined by immunoblotting, or activity, evaluated by analyzing eicosanoids formed from exogenous labeled AA by HPLC. In contrast, RA induced PGI-synthase (PGIS) activity and expression in terms of mRNA and protein determined by real time PCR and western blotting, respectively. Results from experiments with several species of RA and with RAR and RXR antagonists showed that the effect of RA on PGIS expression was mediated by RAR. Actinomycin D and cycloheximide both inhibited RA-induced PGIS expression. Furthermore, RA increased PGIS transcriptional activity in transient transfection assays, an effect that was prevented by a RAR antagonist. These results reinforce the concept that RA could be beneficial for patients with cardiovascular risk.

Cercador de publicacions

Any 21019821983198419851986198719881989199019911992199319941995199619971998199920002001200220032004200520062007200820092010 Autor Aguilar ElisabethBadimon LinaBaldellou MaribelBayés de Luna AntoniBlanco JerónimoCalvayrac OlivierCamino SandraCasaní LauraCatalina PabloCorral Mª ÁngelesCrespo JavierFarré JordiGarcia MaisaGonzález MaríaGuadall AnnaHernández RodrigoHove-Madsen LeifJuan OriolLlorente VicentaLugano RobertaLuque AnaMartínez-González JoseMiguel MartaNadal NuriaPadró TeresaPeña EstherRodríguez CristinaRodriguez RicardoRovira NormaRubio NuriaSegalés EstefaniaSlevin MarkVilahur Gemma Cercar